Cloned (Comment) | Organism |
---|---|
gene mak1, located in a gene cluster, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the His-tagged enzyme in Streptomyces lividans strain 66 using vector pMJP7 | Actinoplanes missouriensis |
gene pep2, sequence comparisons, expression of the His-tagged enzyme in Streptomyces lividans strain 66 using vector pMJP7 | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49800 | - |
x * 49800, His-tagged enzyme, sequence calculation, x * 62000, recombinant His-tagged enzyme, SDS-PAGE | Actinoplanes missouriensis |
52400 | - |
x * 52400, His-tagged enzyme, sequence calculation, x * 53000, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces coelicolor |
53000 | - |
x * 52400, His-tagged enzyme, sequence calculation, x * 53000, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces coelicolor |
62000 | - |
x * 49800, His-tagged enzyme, sequence calculation, x * 62000, recombinant His-tagged enzyme, SDS-PAGE | Actinoplanes missouriensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinoplanes missouriensis | Q7WUM3 | gene mak1 | - |
Streptomyces coelicolor | O54204 | gene pep2 | - |
Streptomyces coelicolor A3(2) | O54204 | gene pep2 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Streptomyces lividans strain 66 by nickel affinity chromatography | Streptomyces coelicolor |
recombinant His-tagged enzyme from Streptomyces lividans strain 66 by nickel affinity chromatography | Actinoplanes missouriensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
144.8 | - |
purified His-tagged recombinant enzyme, pH 7.0, 45°C | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + maltose | maltose cannot be replaced by spectinomycin, streptomycin, kasugamycin, kanamycin, hygromycin, or apramycin as a phosphoryl-group acceptor | Actinoplanes missouriensis | ADP + maltose 1-phosphate | - |
? | |
ATP + maltose | maltotriose, maltotetraose, L-arabinose, inositol, cellobiose, L-lactose, D-mannose, raffinose, or trehalose cannot replace maltose as a phosphorylgroup acceptor in the reaction catalyzed by Pep2. ATP cannot be replaced by other nucleotides as a phosphoryl-group donor | Streptomyces coelicolor | ADP + maltose 1-phosphate | - |
? | |
ATP + maltose | maltotriose, maltotetraose, L-arabinose, inositol, cellobiose, L-lactose, D-mannose, raffinose, or trehalose cannot replace maltose as a phosphorylgroup acceptor in the reaction catalyzed by Pep2. ATP cannot be replaced by other nucleotides as a phosphoryl-group donor | Streptomyces coelicolor A3(2) | ADP + maltose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49800, His-tagged enzyme, sequence calculation, x * 62000, recombinant His-tagged enzyme, SDS-PAGE | Actinoplanes missouriensis |
? | x * 52400, His-tagged enzyme, sequence calculation, x * 53000, recombinant His-tagged enzyme, SDS-PAGE | Streptomyces coelicolor |
Synonyms | Comment | Organism |
---|---|---|
Mak1 | - |
Actinoplanes missouriensis |
Pep2 | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on. ATP cannot be replaced by other nucleotides as a phosphoryl-group donor | Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
physiological function | maltokinase is the enzyme responsible for the ATP-dependent formation of maltose 1-phosphate | Actinoplanes missouriensis |